atcc cell bank Search Results


cell culture bank  (ATCC)
95
ATCC cell culture bank
Cell Culture Bank, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hle jcrb  (JCRB Cell Bank)
90
JCRB Cell Bank hle jcrb
SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and <t>HLE</t> cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection <t>of</t> <t>HuH7</t> cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.
Hle Jcrb, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lm-2-jck  (JCRB Cell Bank)
90
JCRB Cell Bank lm-2-jck
SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and <t>HLE</t> cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection <t>of</t> <t>HuH7</t> cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.
Lm 2 Jck, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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raji, a b-cell lymphoma-derived cells  (JCRB Cell Bank)
90
JCRB Cell Bank raji, a b-cell lymphoma-derived cells
SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and <t>HLE</t> cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection <t>of</t> <t>HuH7</t> cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.
Raji, A B Cell Lymphoma Derived Cells, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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es-2  (JCRB Cell Bank)
90
JCRB Cell Bank es-2
SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and <t>HLE</t> cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection <t>of</t> <t>HuH7</t> cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.
Es 2, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hpaf-ii  (JCRB Cell Bank)
90
JCRB Cell Bank hpaf-ii
SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and <t>HLE</t> cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection <t>of</t> <t>HuH7</t> cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.
Hpaf Ii, supplied by JCRB Cell Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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7g7  (ATCC)
90
ATCC 7g7
SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and <t>HLE</t> cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection <t>of</t> <t>HuH7</t> cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.
7g7, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caki 1  (ATCC)
97
ATCC caki 1
SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and <t>HLE</t> cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection <t>of</t> <t>HuH7</t> cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.
Caki 1, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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crl  (ATCC)
94
ATCC crl
SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and <t>HLE</t> cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection <t>of</t> <t>HuH7</t> cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.
Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal hepatocyte cell line thle 3  (ATCC)
96
ATCC normal hepatocyte cell line thle 3
SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and <t>HLE</t> cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection <t>of</t> <t>HuH7</t> cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.
Normal Hepatocyte Cell Line Thle 3, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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atcc cell bank  (ATCC)
92
ATCC atcc cell bank
SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and <t>HLE</t> cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection <t>of</t> <t>HuH7</t> cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.
Atcc Cell Bank, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chinese hamster ovary cho k1 cells  (ATCC)
92
ATCC chinese hamster ovary cho k1 cells
SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and <t>HLE</t> cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection <t>of</t> <t>HuH7</t> cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.
Chinese Hamster Ovary Cho K1 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and HLE cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection of HuH7 cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.

Journal: Cancers

Article Title: Serum Response Factor (SRF) Drives the Transcriptional Upregulation of the MDM4 Oncogene in HCC

doi: 10.3390/cancers13020199

Figure Lengend Snippet: SRF, ELK1, and ELK4 regulate MDM4 expression in HCC cell lines. ( A ) Increased MDM4 mRNA levels in HepG2 and HLE cell lines following fetal calf serum (FCS) stimulation compared to starved control cells. ( B ) MDM4 mRNA and ( C ) protein levels following siRNA-mediated knockdown of SRF compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( D ) MDM4 mRNA and ( E ) protein levels following siRNA-mediated knockdown of ELK1 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( F ) MDM4 mRNA and ( G ) protein levels following siRNA-mediated knockdown of ELK4 compared to control cells transfected with a scrambled, nonsense siRNA (siNS) in HepG2 and HLE cells. ( H ) siRNA-mediated knockdown of SRF (siSRF_2) prevents FCS-stimulated MDM4 protein upregulation. ( I ) MDM4 mRNA and protein expression 48 h following transfection of HuH7 cells with an SRF-VP16 expression vector compared to mock transfected control cells. Original western blots are shown in . Data are presented as mean ± SEM. Mann–Whitney U test: * p < 0.05, ** p < 0.01, *** p < 0.001. Abbreviations: siNS—scrambled, nonsense siRNA; siSRF_1/_2, siELK1_1/_2, siELK4_1/_2—siRNA 1 and 2 specifically targeting SRF, ELK1, and ELK4, respectively.

Article Snippet: HepG2 cells (ATCC) and Hep3B (ATCC) were cultured in RPMI and MEM medium respectively, while HuH7 (ATCC) and HLE (JCRB) cell lines in DMEM medium (Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Expressing, Control, Knockdown, Transfection, Plasmid Preparation, Western Blot, MANN-WHITNEY